Monday, March 28, 2011
Disease Testing Results
I Think We Should Get Tested...
By analyzing your genes and such through a process called disease gene testing, it is possible to see if you have a disease. In many diseases, early detection is crucial so this technique is incredibly important in today's society.
The first step in this process is to collect DNA to be processed. After swabbing our cheeks, we will use a 95 degree Celsius water bath to break open the cell wall and the nuclear membrane of the cell. Just outside the nucleus are enzymes called DNAse that protect the cell from viral infections. However, this enzyme will also try to attack the DNA we are trying to extract so we must use instagene matrix beads to kill them and protect the DNA. After doing a DNA Polymerase Chain Reaction (PCR) to mass produce the DNA we collect, we will do electrophoresis of the PCR products. Based on the lines that show up, we can tell if we are affected by the disease, carriers, or disease free.
The first step in this process is to collect DNA to be processed. After swabbing our cheeks, we will use a 95 degree Celsius water bath to break open the cell wall and the nuclear membrane of the cell. Just outside the nucleus are enzymes called DNAse that protect the cell from viral infections. However, this enzyme will also try to attack the DNA we are trying to extract so we must use instagene matrix beads to kill them and protect the DNA. After doing a DNA Polymerase Chain Reaction (PCR) to mass produce the DNA we collect, we will do electrophoresis of the PCR products. Based on the lines that show up, we can tell if we are affected by the disease, carriers, or disease free.
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